Detection of human papilloma virus (HPV) in parafin block of cervical cancer patients using multiplex polymerase chain reaction (PCR) and reverse line blot methods
نویسندگان
چکیده
منابع مشابه
detection and typing of human papilloma viruses by nested multiplex polymerase chain reaction assay in cervical cancer
background cervical cancer is the leading cause of death from cancer in under-developed countries. human papilloma virus (hpv) 16 and 18 are the most prevalent types associated with carcinogenesis in the cervix. conventional polymerase chain reaction (pcr), type-specific and consensus primer-based pcr followed by sequencing, restriction fragment length polymorphism (rflp) or hybridization by sp...
متن کاملDetection and Typing of Human Papilloma Viruses by Nested Multiplex Polymerase Chain Reaction Assay in Cervical Cancer
BACKGROUND Cervical cancer is the leading cause of death from cancer in under-developed countries. Human papilloma virus (HPV) 16 and 18 are the most prevalent types associated with carcinogenesis in the cervix. Conventional Polymerase Chain Reaction (PCR), type-specific and consensus primer-based PCR followed by sequencing, Restriction Fragment Length Polymorphism (RFLP) or hybridization by sp...
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متن کاملDetection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction
Human papilloma virus (HPV) DNA sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. Amplification of a HPV DNA sequence before its detection with a cDNA probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue se...
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ژورنال
عنوان ژورنال: Medicina Clínica Práctica
سال: 2021
ISSN: 2603-9249
DOI: 10.1016/j.mcpsp.2021.100225